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Histological characterization of oocyte developmental stages of suruvi Steindachneridion scriptum kept in captivity Animal Sciences
Abreu, Mariana Roza; Garcia, Patrícia; Zaniboni-Filho, Evoy.
Stages of oocyte development were described for suruvi females (Steindachneridion scriptum) kept in captivity. Oocytes of 21 mature or maturing females and ovaries of 12 immature females were fixed in Karnovsky solution for 4 and 12 hours, respectively. After, they were processed for routine histology, embedded in paraffin and stained with hematoxylin-eosin. Slides were examined under a light microscope and the oocytes were measured using the LAS EZ software, using the average between the largest and the smallest diameter of each oocyte for representation. There were six types of oocytes: Chromatin nucleolus: found in immature females, with average diameter of 9.5 ± 4.46 μm; Perinucleolar: present in all developmental stages with average diameter of 67.6 ±...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.05.04.00-2 histology; Oogenesis; Fish reproduction; Vitellogenesis..
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/27570
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Induced reproduction of dourado (Salminus brasiliensis): fertilization with sperm cryopreserved in ACP®-104 Animal Sciences
Zanandrea, Ana Carolina Volpato; Weingartner, Marcos; Zaniboni-Filho, Evoy.
Experiments were conducted to improve the fertilization rate of cryopreserved semen of dourado, Salminus brasiliensis. The experiment tested two cryoprotectant solutions at different semen: cryoprotectant ratios (1:5, 1:15, 1:25 and 1:50). The standard solution for the species (mixture of dimethyl sulfoxide, glucose, egg yolk and distilled water) was compared to a 350 mOsm ACP®-104 solution, which is composed of powdered coconut water diluted in distilled water and methylglycol. Differences between the dilutions tested were significant only for ACP®. The fertilization potential by using the standard solution at the lowest dilution (1:5) is equated when the sperm is diluted in ACP® at 1:25 or 1:50. These results show that the standard solution is the most...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.06.03.04-3 cryopreservation; Sperm; Fertilization; Cryoprotectant; Coconut water.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/28838
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